In our class, we are looking at the relationship between fruits, breads, and microbes. We are studying the history of the bread microbial prevalence compared to that of breads in that culture.
The only prior experience with DNA sequencing I have is experience with electrophoresis in Biology courses I have taken.
Both Sanger sequencing and NGS involve the addition of fluorescent nucleotides to a DNA template strand. NGS, as our Next Generation Sequencing technology, can perform millions of fragments simultaneously, and not only increases the speed of genomic coverage, but allows a higher sensitivity to variants. This also allows the detection of variants and rarities to occur much faster.
We are using the Illumina sequencing workflow to sequence through synthesis. We do this (I believe) by taking fragments and adding to the ends, hybridizing them. Following this, we cluster them together, and allow these to create template strands. There are further specifics such as bridges, and through the entire process we can separate differences based off of introduced factors.
https://www.illumina.com/science/technology/next-generation-sequencing/ngs-vs-sanger-sequencing.html?langsel=/us/
https://www.thermofisher.com/us/en/home/life-science/sequencing/sequencing-learning-center/next-generation-sequencing-information/ngs-basics/what-is-next-generation-sequencing.html
https://www.khanacademy.org/science/ap-biology/gene-expression-and-regulation/biotechnology/v/dna-sequencing